A hybrid de novo genome assembly of the honeybee, Apis mellifera, with chromosome-length scaffolds.

ABSTRACT:

Background

The ability to generate long sequencing reads and access long-range linkage information is revolutionizing the quality and completeness of genome assemblies. Here we use a hybrid approach that combines data from four genome sequencing and mapping technologies to generate a new genome assembly of the honeybee Apis mellifera. We first generated contigs based on PacBio sequencing libraries, which were then merged with linked-read 10x Chromium data followed by scaffolding using a BioNano optical genome map and a Hi-C chromatin interaction map, complemented by a genetic linkage map.

Results

Each of the assembly steps reduced the number of gaps and incorporated a substantial amount of additional sequence into scaffolds. The new assembly (Amel_HAv3) is significantly more

SUBMITTER: Wallberg A 

PROVIDER: S-EPMC6454739 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature