Identification and Expression Patterns of Three Vitellogenin Genes and Their Roles in Reproduction of the Alligatorweed Flea Beetle Agasicles hygrophila (Coleoptera: Chrysomelidae).

ABSTRACT: The alligatorweed flea beetle Agasicles hygrophila is an insect used for biological control of the aquatic weed Alternanthera philoxeroides (alligatorweed). Because these insects are oviparous, synthesis, and transportation of yolk proteins is integral to reproduction. Vitellin, the chief protein constituent in egg yolk, is mainly synthesized in the fat body and its synthesis is regulated by the transcript levels of Vitellogenin (Vg). In our study, we first cloned and characterized three Vg genes from A. hygrophila and quantified the expression levels of these Vgs in different tissues and developmental stages by RT-qPCR. Analysis of the full-length cDNA sequences of the three A. hygrophila Vg genes revealed that the open reading frame

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6454870 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature