Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA.

ABSTRACT: Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.

SUBMITTER: Valencia LE 

PROVIDER: S-EPMC6454893 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature