Replication and virulence in pigs of the first African swine fever virus isolated in China.

ABSTRACT: African swine fever (ASF) entered China in August 2018 and rapidly spread across the entire country, severely threatening the Chinese domestic pig population, which accounts for more than 50% of the pig population worldwide. In this study, an ASFV isolate, Pig/Heilongjiang/2018 (Pig/HLJ/18), was isolated in primary porcine alveolar macrophages (PAMs) from a pig sample from an ASF outbreak farm. The isolate was characterized by using the haemadsorption (HAD) test, Western blotting and immunofluorescence, and electronic microscopy. Phylogenetic analysis of the viral p72 gene revealed that Pig/HLJ/18 belongs to Genotype II. Infectious titres of virus propagated in primary PAMs and pig marrow macrophages were as high as 10^7.2 HAD₅₀/ml. Specific-pathogen-free pigs intramus

SUBMITTER: Zhao D 

PROVIDER: S-EPMC6455124 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature