Dietary Protein Restriction Reprograms Tumor-Associated Macrophages and Enhances Immunotherapy.

ABSTRACT:

Purpose

Diet and healthy weight are established means of reducing cancer incidence and mortality. However, the impact of diet modifications on the tumor microenvironment and antitumor immunity is not well defined. Immunosuppressive tumor-associated macrophages (TAMs) are associated with poor clinical outcomes and are potentially modifiable through dietary interventions. We tested the hypothesis that dietary protein restriction modifies macrophage function toward antitumor phenotypes.

Experimental design

Macrophage functional status under different tissue culture conditions and in vivo was assessed by Western blot, immunofluorescence, qRT-PCR, and cytokine array analyses. Tumor growth in the context of protein or amino acid (AA) restriction and immunotherapy, namely, a

SUBMITTER: Orillion A 

PROVIDER: S-EPMC6455918 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature