Characterization of Human Adrenal Steroidogenesis During Fetal Development.

ABSTRACT:

Context

The endocrine function of human fetal adrenals (HFAs) is activated already during first trimester, but adrenal steroidogenesis during fetal life is not well characterized.

Objective

This study aimed to investigate HFA steroidogenesis by analyzing adrenal glands from first and second trimesters.

Design and setting

Male and female HFA from gestational weeks (GWs) 8 to 19 were examined, including a total of 101 samples from 83 fetuses.

Main outcome measure(s)

Expression level of steroidogenic genes and protein expression/localization were determined by quantitative PCR and immunohistochemistry, respectively, and intra-adrenal steroid levels were quantified by LC-MS/MS.

Results

Transcriptional levels of StAR, CYP11A1, CYP17A1, CYP21A2, CYP11B1/2, and

SUBMITTER: Melau C 

PROVIDER: S-EPMC6456011 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature