A Platform for Generation of Chamber-Specific Cardiac Tissues and Disease Modeling.

ABSTRACT: Tissue engineering using cardiomyocytes derived from human pluripotent stem cells holds a promise to revolutionize drug discovery, but only if limitations related to cardiac chamber specification and platform versatility can be overcome. We describe here a scalable tissue-cultivation platform that is cell source agnostic and enables drug testing under electrical pacing. The plastic platform enabled on-line noninvasive recording of passive tension, active force, contractile dynamics, and Ca²⁺ transients, as well as endpoint assessments of action potentials and conduction velocity. By combining directed cell differentiation with electrical field conditioning, we engineered electrophysiologically distinct atrial and ventricular tissues with chamber-specific drug responses and gene

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6456036 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature