Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites.

ABSTRACT: Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome assembly is a selective process driven by RNA-RNA interactions and is hypothesized to lead to discrete punctate structures scattered through the cytosol. Here, we show that contrary to the accepted view, formation of these structures precedes RNA-RNA interactions among distinct viral ribonucleoproteins (vRNPs), as they assemble in cells expressing only one vRNP type. We demonstrate that these viral inclusions display characteristics of liquid organelles, segregating from the cytosol without a delimitating membrane, dynamically exchanging material and adapting fast to environmental changes. We provide evidence that viral inclusions develop close to endoplasmic

SUBMITTER: Alenquer M 

PROVIDER: S-EPMC6456594 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature