Improving in vitro photodynamic therapy through the development of a novel iron chelating aminolaevulinic acid prodrug.

ABSTRACT:

Background

Photodynamic therapy (PDT) is a light activated drug therapy that can be used to treat a number of cancers and precancers. It is particularly useful in its topical form in dermatology but improvement of efficacy is required to widen its application.

Methods

An ester between aminolaevulinic acid (ALA) and CP94 was synthesised (AP2-18) and experimentally evaluated to determine whether protoporphyrin IX (PpIX)-induced PDT effectiveness could be improved. A biological evaluation of AP2-18 was conducted in cultured human primary cells with both PpIX fluorescence and cell viability (as determined via the neutral red assay) being assessed in comparison to the PpIX prodrugs normally utilised in clinical practice (aminolaevulinic acid (ALA) or its methyl ester (MAL)) eithe

SUBMITTER: Curnow A 

PROVIDER: S-EPMC6456724 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature