IsoProt: A Complete and Reproducible Workflow To Analyze iTRAQ/TMT Experiments.

ABSTRACT: Reproducibility has become a major concern in biomedical research. In proteomics, bioinformatic workflows can quickly consist of multiple software tools each with its own set of parameters. Their usage involves the definition of often hundreds of parameters as well as data operations to ensure tool interoperability. Hence, a manuscript's methods section is often insufficient to completely describe and reproduce a data analysis workflow. Here we present IsoProt: A complete and reproducible bioinformatic workflow deployed on a portable container environment to analyze data from isobarically labeled, quantitative proteomics experiments. The workflow uses only open source tools and provides a user-friendly and interactive browser interface to configure and execute the different operations. Onc

SUBMITTER: Griss J 

PROVIDER: S-EPMC6456869 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature