Controlling a burn: outer-sphere gating of hydroxylamine oxidation by a distal base in cytochrome P460.

ABSTRACT: Ammonia oxidizing bacteria (AOB) use the cytotoxic, energetic molecule hydroxylamine (NH₂OH) as a source of reducing equivalents for cellular respiration. Despite disproportionation or violent decomposition being typical outcomes of reactions of NH₂OH with iron, AOB and anammox heme P460 proteins including cytochrome (cyt) P460 and hydroxylamine oxidoreductase (HAO) effect controlled, stepwise oxidation of NH₂OH to nitric oxide (NO). Curiously, a recently characterized cyt P460 variant from the AOB Nitrosomonas sp. AL212 is able to form all intermediates of cyt P460 catalysis, but is nevertheless incompetent for NH₂OH oxidation. We now show via site-directed mutagenesis, activity assays, spectroscopy, and structural biology that thi

SUBMITTER: Smith MA 

PROVIDER: S-EPMC6457333 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature