Complex Coacervation-Integrated Hybrid Nanoparticles Increasing Plasmid DNA Delivery Efficiency in Vivo.

ABSTRACT: Many polycation-based gene delivery vehicles have limited in vivo transfection efficiency because of their excessive exterior positive charges and/or PEGylation, both of which could result in premature dissociation and poor cellular uptake and trafficking. Here, we reported novel hybrid PEGylated nanoparticles (HNPs) that are composed of (a) poly(ethylene glycol)-b-poly(aspartate)-adamantane (PEG-P(asp)-Ad) constituting the outer PEG layer to provide colloidal stability; (b) poly(ethylenimine)_10K (PEI_10K) forming complex coacervate with P(asp) as the cross-linked cage preventing premature dissociation; (c) cyclodextrin-decorated PEI_10K (PEI_10K-CD) forming the core with reporter plasmid DNA (pDNA). These HNPs exhibited an increased stability and h

SUBMITTER: Li Y 

PROVIDER: S-EPMC6457453 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature