RNAi-mediated knockdown of daf-12 in the model parasitic nematode Strongyloides ratti.

ABSTRACT: The gene daf-12 has long shown to be involved in the dauer pathway in Caenorhabditis elegans (C. elegans). Due to the similarities of the dauer larvae of C. elegans and infective larvae of certain parasitic nematodes such as Strongyloides spp., this gene has also been suspected to be involved in the development of infective larvae. Previous research has shown that the application of dafachronic acid, the steroid hormone ligand of DAF-12 in C. elegans, affects the development of infective larvae and metabolism in Strongyloides. However, a lack of tools for either forward or reverse genetics within Strongyloides has limited studies of gene function within these important parasites. After determining whether Strongyloides had the requisite proteins for RNAi, we developed and report here the f

SUBMITTER: Dulovic A 

PROVIDER: S-EPMC6457571 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature