Cellular and Chemical Gradients to Engineer the Meniscus-to-Bone Insertion.

ABSTRACT: Tissue-engineered menisci hold promise as an alternative to allograft procedures but require a means of robust fixation to the native bone. The insertion of the meniscus into bone is critical for meniscal function and inclusion of a soft tissue-to-bone interface in a tissue engineered implant can aid in the fixation process. The native insertion is characterized by gradients in composition, tissue architecture, and cellular phenotype, which are all difficult to replicate. In this study, a soft tissue-to-bone interface is tissue engineered with a cellular gradient of fibrochondrocytes and mesenchymal stem cells and subjected to a biochemical gradient through a custom media diffusion bioreactor. These constructs, consisting of interpenetrating collagen and boney regions, display improved mec

SUBMITTER: Iannucci LE 

PROVIDER: S-EPMC6458090 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature