Dynamic Interactions between Lipid-Tethered DNA and Phospholipid Membranes.

ABSTRACT: Lipid-anchored DNA can attach functional cargo to bilayer membranes in DNA nanotechnology, synthetic biology, and cell biology research. To optimize DNA anchoring, an understanding of DNA-membrane interactions in terms of binding strength, extent, and structural dynamics is required. Here we use experiments and molecular dynamics (MD) simulations to determine how the membrane binding of cholesterol-modified DNA depends on electrostatic and steric factors involving the lipid headgroup charge, duplexed or single-stranded DNA, and the buffer composition. The experiments distinguish between free and membrane vesicle-bound DNA and thereby reveal the surface density of anchored DNA and its binding affinity, something which had previously not been known. The K_d values range from 8.5 ±

SUBMITTER: Arnott PM 

PROVIDER: S-EPMC6458106 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature