Characterization of cytoplasmic viscosity of hundreds of single tumour cells based on micropipette aspiration.

ABSTRACT: Cytoplasmic viscosity (μ _c) is a key biomechanical parameter for evaluating the status of cellular cytoskeletons. Previous studies focused on white blood cells, but the data of cytoplasmic viscosity for tumour cells were missing. Tumour cells (H1299, A549 and drug-treated H1299 with compromised cytoskeletons) were aspirated continuously through a micropipette at a pressure of -10 or -5 kPa where aspiration lengths as a function of time were obtained and translated to cytoplasmic viscosity based on a theoretical Newtonian fluid model. Quartile coefficients of dispersion were quantified to evaluate the distributions of cytoplasmic viscosity within the same cell type while neural network-based pattern recognitions were used to classify different cell types based on cytoplasm

SUBMITTER: Wang K 

PROVIDER: S-EPMC6458365 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature