Prevalence and Phylogenetic Analysis of Mycoplasma Synoviae Strains Isolated from Polish Chicken Layer Flocks.

ABSTRACT:

Introduction

Mycoplasma synoviae (MS) is a chicken pathogen of major economic importance.

Material and methods

Between 2010 and 2016, 906 commercial layer chicken flocks in Poland were examined for MS, and the phylogenetic relationship among the strains was established. Regionally dispersed samples were collected and tested with the use of real-time PCR to detect the 16S-23S intergenic spacer region. Positive samples were also tested with LAMP and conventional PCR to detect the vlhA gene.

Results

MS genetic material was detected in 265 (29%) of the tested flocks by real-time PCR, in 227 by the LAMP method and in 202 (22%) by conventional PCR. The by-year percentage of positive samples began at 34% in 2010, rose to 44% in 2012, and declined to 29% in 2016

SUBMITTER: Kursa O 

PROVIDER: S-EPMC6458562 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature