Construction of a bacterial surface display system based on outer membrane protein F.

ABSTRACT:

Background

Bacterial surface display systems were developed to surface expose heterologous proteins or peptides for different applications, such as peptide libraries screening and live bacterial vaccine design. Various outer membrane proteins, such as outer membrane protein A (OmpA), OmpC and outer membrane pore protein E precursor (PhoE), have been used as carriers for surface display, fused to the proteins or peptides of interest in Gram-negative bacteria. Here, we investigated the utility of constitutively expressed OmpF for the display of foreign immune epitopes on the Escherichia coli cell surface and then compared it with plasmid-induced expression of OmpF and OmpC.

Results

Enhanced expression of OmpF was linked to a mutation in the OmpF promoter sequence. This mutatio

SUBMITTER: Chen T 

PROVIDER: S-EPMC6458713 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature