In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells.

ABSTRACT: Amniotic epithelial cells (AECs), an emerging source of extrafoetal stem cells, have recently attracted attention for their great regenerative potential. Since AEC amplifications are accompanied by the loss of their native epithelial phenotype and by the progressive reduction of relevant biological properties, the issue to be addressed is the development of effective culture protocols. In this context, recently, it has been demonstrated that progesterone (P₄) supplementation during ovine AEC (oAEC) expansion could prevent the undesirable epithelial-mesenchymal transition (EMT). In contrast, there is no information to date on the role of the other pregnancy steroids in culture. With this aim, the present study has been designed to clarify the impact of estradiol (E₂),

SUBMITTER: Mauro A 

PROVIDER: S-EPMC6458847 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature