Biophysical Characterization Platform Informs Protein Scaffold Evolvability.

ABSTRACT: Evolving specific molecular recognition function of proteins requires strategic navigation of a complex mutational landscape. Protein scaffolds aid evolution via a conserved platform on which a modular paratope can be evolved to alter binding specificity. Although numerous protein scaffolds have been discovered, the underlying properties that permit binding evolution remain unknown. We present an algorithm to predict a protein scaffold's ability to evolve novel binding function based upon computationally calculated biophysical parameters. The ability of 17 small proteins to evolve binding functionality across seven discovery campaigns was determined via magnetic activated cell sorting of 1010 yeast-displayed protein variants. Twenty topological and biophysical properties were calculated fo

SUBMITTER: Golinski AW 

PROVIDER: S-EPMC6458986 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature