Upregulation of FOXP4 in breast cancer promotes migration and invasion through facilitating EMT.

ABSTRACT:

Background

Family of forkhead box transcription factors has been found to play key roles in multiple types of cancer.

Materials and methods

Our study is to decipher the effects of FOXP4 in human breast cancer (BC). Quantitative real-time polymerase chain reaction and Western blot analyses were performed to determine the mRNA and protein expressions of FOXP4 in BC tissue samples and cell lines. The gain and loss of function assay were used to explore the detailed roles of FOXP4 in breast cell lines, including MDA-MB-231 and MCF-7 cells. Its effect on BC growth, migration, and invasion were evaluated by colony formation assay, CCK-8 assay, wound-healing assay, and transwell invasion assay, respectively.

Results

Our findings revealed that FOXP4 promotes cell proliferatio

SUBMITTER: Ma T 

PROVIDER: S-EPMC6459146 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature