Protein kinase D mediates inflammatory responses of human placental macrophages to Group B Streptococcus.

ABSTRACT:

Problem

During pregnancy, Group B Streptococcus (GBS) can infect fetal membranes to cause chorioamnionitis, resulting in adverse pregnancy outcomes. Macrophages are the primary resident phagocyte in extraplacental membranes. Protein kinase D (PKD) was recently implicated in mediating pro-inflammatory macrophage responses to GBS outside of the reproductive system. This work aimed to characterize the human placental macrophage inflammatory response to GBS and address the extent to which PKD mediates such effects.

Method

Primary human placental macrophages were infected with GBS in the presence or absence of a specific, small molecule PKD inhibitor, CRT 0066101. Macrophage phenotypes were characterized by evaluating gene expression, cytokine release, assembly of the NLRP3 infla

SUBMITTER: Sutton JA 

PROVIDER: S-EPMC6459189 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature