Oocyte-specific deletion of N-WASP does not affect oocyte polarity, but causes failure of meiosis II completion.

ABSTRACT:

Study question

There is an unexplored physiological role of N-WASP (neural Wiskott-Aldrich syndrome protein) in oocyte maturation that prevents completion of second meiosis.

Summary answer

In mice, N-WASP deletion did not affect oocyte polarity and asymmetric meiotic division in first meiosis, but did impair midbody formation and second meiosis completion.

What is known already

N-WASP regulates actin dynamics and participates in various cell activities through the RHO-GTPase-Arp2/3 (actin-related protein 2/3 complex) pathway, and specifically the Cdc42 (cell division cycle 42)-N-WASP-Arp2/3 pathway. Differences in the functions of Cdc42 have been obtained from in vitro compared to in vivo studies.

Study design, samples/materials, methods

By conditional knockout

SUBMITTER: Wang ZB 

PROVIDER: S-EPMC6459223 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature