Genomic Reorganization of Lamin-Associated Domains in Cardiac Myocytes Is Associated With Differential Gene Expression and DNA Methylation in Human Dilated Cardiomyopathy.

ABSTRACT:

Rationale

LMNA (Lamin A/C), a nuclear membrane protein, interacts with genome through lamin-associated domains (LADs) and regulates gene expression. Mutations in the LMNA gene cause a diverse array of diseases, including dilated cardiomyopathy (DCM). DCM is the leading cause of death in laminopathies.

Objective

To identify LADs and characterize their associations with CpG methylation and gene expression in human cardiac myocytes in DCM.

Methods and results

LMNA chromatin immunoprecipitation-sequencing, reduced representative bisulfite sequencing, and RNA-sequencing were performed in 5 control and 5 LMNA-associated DCM hearts. LADs were identified using enriched domain detector program. Genome-wide 331±77 LADs with an average size of 2.1±1.5 Mbp were identified in cont

SUBMITTER: Cheedipudi SM 

PROVIDER: S-EPMC6459729 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature