The invasive cell coat at the microsporidian Trachipleistophora hominis-host cell interface contains secreted hexokinases.

ABSTRACT: Microsporidia are obligate intracellular parasites causing significant disease in humans and economically important animals. In parallel to their extreme genetic reduction, Microsporidia have evolved novel mechanisms for exploiting host metabolism. A number of microsporidians confer secretion of otherwise cytosolic proteins by coding for signal peptides that direct entry into the endoplasmic reticulum. The human pathogen Trachipleistophora hominis encodes for four hexokinases, three of which have signal peptides at the N-terminus. Here, we localized hexokinase 2 and hexokinase 3 through developmental stages of T. hominis using light and electron microscopy. Both proteins were concentrated in an extracellular coat previously termed the plaque matrix (PQM). The PQM (containing hexokinases) w

SUBMITTER: Ferguson S 

PROVIDER: S-EPMC6460350 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature