Derivation of adult canine intestinal organoids for translational research in gastroenterology.

ABSTRACT:

Background

Large animal models, such as the dog, are increasingly being used for studying diseases including gastrointestinal (GI) disorders. Dogs share similar environmental, genomic, anatomical, and intestinal physiologic features with humans. To bridge the gap between commonly used animal models, such as rodents, and humans, and expand the translational potential of the dog model, we developed a three-dimensional (3D) canine GI organoid (enteroid and colonoid) system. Organoids have recently gained interest in translational research as this model system better recapitulates the physiological and molecular features of the tissue environment in comparison with two-dimensional cultures.

Results

Organoids were derived from tissue of more than 40 healthy dogs and dogs with GI

SUBMITTER: Chandra L 

PROVIDER: S-EPMC6460554 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature