Immunogenicity analysis of genetically conserved segments in Plasmodium ovale merozoite surface protein-8.

ABSTRACT:

Background

Plasmodium ovale is widely distributed across tropical regions and has two closely related but distinct species, namely P. ovale curtisi and P. ovale wallikeri. Combining genetic characterization with the immunogenicity of merozoite surface protein-8 (MSP-8) supports considering MSP-8 as a candidate target for blood-stage vaccines against malaria. However, no previous studies have focused on characterizing the genetic diversity and immunogenicity of PoMSP-8.

Methods

Blood samples were collected from 42 patients infected with P. ovale. The patients were migrant workers returning to the Jiangsu Province from Africa; genomic DNA was extracted from their blood samples for sequencing and protein expression. The recombinant PoMSP-8 (rPoMSP-8) proteins were expressed and

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6460738 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature