Quantification of serine protease HtrA molecules secreted by the foodborne pathogen Campylobacter jejuni.

ABSTRACT:

Background

Campylobacter jejuni is a major food-borne pathogen and a worldwide health threat. Utilizing different virulence factors, C. jejuni invades the host's intestinal epithelial cell layer. One important factor in this process is the serine protease HtrA, which is secreted into the extracellular space, and helps the bacteria to transmigrate across the gut epithelium by cleaving various cell-cell adhesion proteins. The aim of the present study is to quantify the amount of HtrA molecules secreted per bacterial cell in liquid culture and during infection.

Results

HtrA protein purification and quantitative Western blotting were used to determine the number of HtrA molecules secreted by two C. jejuni model strains, 11168 and 81-176, in liquid culture du

SUBMITTER: Neddermann M 

PROVIDER: S-EPMC6460743 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature