Conditioned Medium of Human Menstrual Blood-Derived Endometrial Stem Cells Protects Against MPP⁺-Induced Cytotoxicity in vitro.

ABSTRACT: Mesenchymal stem cells (MSCs) showed the potential to treat Parkinson's disease (PD). However, it is unknown whether the conditioned medium of human menstrual blood-derived endometrial stem cells (MenSCs-CM) has the function to alleviate syndromes of PD. In this study, human neuroblastoma SH-SY5Y cells were exposed to neurotoxicant 1-methyl-4-phenylpyridinium (MPP⁺) for inducing a range of response characteristics of PD. After culturing this cell model with 24 h/48 h collected MenSCs-CM for different days, cell viability, pro-inflammation cytokines, mitochondrial membrane potential (ΔΨm), oxidative stress, and cell apoptosis were detected. Finally, protein assay was performed to detect 12 kinds of neurotrophic factors inside MenSCs-CM. Our results showed that MPP⁺ cau

SUBMITTER: Li H 

PROVIDER: S-EPMC6460823 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature