Location analysis of 8-oxo-7,8-dihydroguanine in DNA by polymerase-mediated differential coding.

ABSTRACT: Accumulating lines of evidence indicate that reactive oxygen species (ROS) are important signalling molecules for various cellular processes. 8-Oxo-7,8-dihydroguanine (OG) is a prominent oxidative modification formed in DNA by ROS. Recently, it has been proposed that OG may have regulatory and possibly epigenetic-like properties in modulating gene expression by interfering with transcription components or affecting the formation of G-quadruplex structures. Deciphering the molecular mechanisms of OG on regulation of gene expression requires uncovering the location of OG on genome. In the current study, we characterized two commercially available DNA polymerases, Bsu DNA polymerase (Bsu Pol) and Tth DNA polymerase (Tth Pol), which can selectively incorporate adeni

SUBMITTER: Tang F 

PROVIDER: S-EPMC6460952 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature