Integrating genomic resources to present full gene and putative promoter capture probe sets for bread wheat.

ABSTRACT:

Background

Whole-genome shotgun resequencing of wheat is expensive because of its large, repetitive genome. Moreover, sequence data can fail to map uniquely to the reference genome, making it difficult to unambiguously assign variation. Resequencing using target capture enables sequencing of large numbers of individuals at high coverage to reliably identify variants associated with important agronomic traits. Previous studies have implemented complementary DNA/exon or gene-based probe sets in which the promoter and intron sequence is largely missing alongside newly characterized genes from the recent improved reference sequences.

Results

We present and validate 2 gold standard capture probe sets for hexaploid bread wheat, a gene and a putative promoter capture, which are des

SUBMITTER: Gardiner LJ 

PROVIDER: S-EPMC6461119 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature