Immunoregulation of macrophages by dynamic ligand presentation via ligand-cation coordination.

ABSTRACT: Macrophages regulate host responses to implants through their dynamic adhesion, release, and activation. Herein, we employ bisphosphonate (BP)-coated gold nanoparticle template (BNP) to direct the swift and convertible formation of Mg²⁺-functional Mg²⁺-BP nanoparticle (NP) on the BP-AuNP surface via reversible Mg²⁺-BP coordination, thus producing (Mg²⁺-BP)-Au dimer (MgBNP). Ethylenediaminetetraacetic acid-based Mg²⁺ chelation facilitates the dissolution of Mg²⁺-BP NP, thus enabling the reversion of the MgBNP to the BNP. This convertible nanoassembly incorporating cell-adhesive Mg²⁺ moieties directs reversible attachment and detachment of macrophages by BP and EDTA, without physical scraping or trypsin that could d

SUBMITTER: Kang H 

PROVIDER: S-EPMC6461616 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature