An in vivo wound healing model for the characterization of the angiogenic process and its modulation by pharmacological interventions.

ABSTRACT: Angiogenesis during wound healing is essential for tissue repair and also affected during cancer treatment by anti-angiogenic drugs. Here, we introduce a minimally invasive wound healing model in the mouse ear to assess angiogenesis with high spatiotemporal resolution in a longitudinal manner in vivo using two-photon microscopy in mice expressing GCaMP2 in arterial endothelial cells. The development of vascular sprouts occurred in a highly orchestrated manner within a time window of 8 days following wounding. Novel sprouts developed exclusively from the distal stump of the transsected arteries, growing towards the proximal arterial stump. This was in line with the incidence of Ca²⁺ transients in the arterial endothelial cells, most probably a result of VEGF stimulation, which we

SUBMITTER: Schneider MK 

PROVIDER: S-EPMC6461656 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature