A comprehensive proteomics-based interaction screen that links DYRK1A to RNF169 and to the DNA damage response.

ABSTRACT: Dysregulation of the DYRK1A protein kinase has been associated with human disease. On the one hand, its overexpression in trisomy 21 has been linked to certain pathological traits of Down syndrome, while on the other, inactivating mutations in just one allele are responsible for a distinct yet rare clinical syndrome, DYRK1A haploinsufficiency. Moreover, altered expression of this kinase may also provoke other human pathologies, including cancer and diabetes. Although a few DYRK1A substrates have been described, its upstream regulators and downstream targets are still poorly understood, an information that could shed light on the functions of DYRK1A in the cell. Here, we carried out a proteomic screen using antibody-based affinity purification coupled to mass spectrometry to identify protei

SUBMITTER: Roewenstrunk J 

PROVIDER: S-EPMC6461666 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature