Reduction in polyamine catabolism leads to spermine-mediated airway epithelial injury and induces asthma features.

ABSTRACT: BACKGROUND:Airway epithelial injury is a crucial component of acute and severe asthma pathogenesis and a promising target for treatment of refractory asthma. However, the underlying mechanism of epithelial injury remains poorly explored. Although high levels of polyamines, mainly spermine, have been found in asthma and comorbidity, their role in airway epithelial injury and the cause of their altered levels in asthma have not been explored. METHODS:We measured key polyamine metabolic enzymes in lung samples from normal and asthmatic subjects and in mice with OVA-induced allergic airway inflammation (AAI). Polyamine metabolism was modulated using pharmacologic/genetic modulators. Epithelial stress and apoptosis were measured by TSLP levels and TUNEL assay, respectively. RESULTS:We found los

SUBMITTER: Jain V 

PROVIDER: S-EPMC6461714 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature