Targeting Notch1 and IKKα Enhanced NF-κB Activation in CD133⁺ Skin Cancer Stem Cells.

ABSTRACT: Cancer stem-like cells are hypothesized to be the major tumor-initiating cell population of human cutaneous squamous cell carcinoma (cSCC), but the landscape of molecular alterations underpinning their signaling and cellular phenotypes as drug targets remains undefined. In this study, we developed an experimental pipeline to isolate a highly enriched CD133⁺CD31^-CD45^-CD61^-CD24^- (CD133⁺) cell population from primary cSCC specimens by flow cytometry. The CD133⁺ cells show enhanced stem-like phenotypes, which were verified by spheroid and colony formation in vitro and tumor generation in vivo Gene expression profiling of CD133^+/- cells was compared and validated, and differentially expressed g

SUBMITTER: Quan XX 

PROVIDER: S-EPMC6461743 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature