Effect of fecal microbiota transplantation route of administration on gut colonization and host response in preterm pigs.

ABSTRACT: This study examined gut colonization patterns and host responses to fecal microbiota transplantation (FMT) by different administration routes after preterm birth. In two separate experiments, cesarean-delivered, preterm pigs were administered combined oral + rectal, or exclusively rectal donor feces, and compared with saline controls. After 5 days, stomach and colon bacterial compositions were determined by 16S rRNA gene amplicon sequencing, and organic acid metabolites measured. Further, gut pathology, mucosa bacterial adherence, and goblet cell density were assessed. FMT increased the relative abundance of obligate anaerobes in the colon without affecting total bacterial load. Bacteroides colonized recipients despite low abundance in the donor feces, whereas highly abundant Prevotella an

SUBMITTER: Brunse A 

PROVIDER: S-EPMC6461782 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature