Long-term succession in a coal seam microbiome during in situ biostimulation of coalbed-methane generation.

ABSTRACT: Despite the significance of biogenic methane generation in coal beds, there has never been a systematic long-term evaluation of the ecological response to biostimulation for enhanced methanogenesis in situ. Biostimulation tests in a gas-free coal seam were analysed over 1.5 years encompassing methane production, cell abundance, planktonic and surface associated community composition and chemical parameters of the coal formation water. Evidence is presented that sulfate reducing bacteria are energy limited whilst methanogenic archaea are nutrient limited. Methane production was highest in a nutrient amended well after an oxic preincubation phase to enhance coal biofragmentation (calcium peroxide amendment). Compound-specific isotope analyses indicated the predominance of acetoclastic methan

SUBMITTER: Beckmann S 

PROVIDER: S-EPMC6461797 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature