Macrophage migration inhibitory factor facilitates prostaglandin E₂ production of astrocytes to tune inflammatory milieu following spinal cord injury.

ABSTRACT:

Background

Astrocytes have been shown to produce several pro- and anti-inflammatory cytokines to maintain homeostasis of microenvironment in response to vast array of CNS insults. Some inflammation-related cytokines are responsible for regulating such cell events. Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that can be inducibly expressed in the lesioned spinal cord. Unknown is whether MIF can facilitate the production of immunosuppressive factors from astrocytes to tune milieu following spinal cord injury.

Methods

Following establishment of contusion SCI rat model, correlation of PGE₂ synthesis-related protein levels with that of MIF was assayed by Western blot. ELISA assay was used to detect production of PGE₂, TNF-α, IL

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6461812 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature