Tumour-reactive T cell subsets in the microenvironment of ovarian cancer.

ABSTRACT:

Background

Solid malignancies are frequently infiltrated with T cells. The success of adoptive cell transfer (ACT) with expanded tumour-infiltrating lymphocytes (TILs) in melanoma warrants its testing in other cancer types. In this preclinical study, we investigated whether clinical-grade TILs could be manufactured from ovarian cancer (OC) tumour specimens.

Methods

Thirty-four tumour specimens were obtained from 33 individual patients with OC. TILs were analysed for phenotype, antigen specificity and functionality.

Results

Minimally expanded TILs (Young TILs) were successfully established from all patients. Young TILs contained a high frequency of CD3⁺ cells with a variable CD4/CD8 ratio. TILs could be expanded to clinical numbers. Importantly, recognition

SUBMITTER: Westergaard MCW 

PROVIDER: S-EPMC6461863 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature