Tumour cell blebbing and extracellular vesicle shedding: key role of matrikines and ribosomal protein SA.

ABSTRACT:

Background

Carcinogenesis occurs in elastin-rich tissues and leads to local inflammation and elastolytic proteinase release. This contributes to bioactive matrix fragment (Matrikine) accumulation like elastin degradation products (EDP) stimulating tumour cell invasive and metastatic properties. We previously demonstrate that EDPs exert protumoural activities through Hsp90 secretion to stabilised extracellular proteinases.

Methods

EDP influence on cancer cell blebbing and extracellular vesicle shedding were examined with a videomicroscope coupled with confocal Yokogawa spinning disk, by transmission electron microscopy, scanning electron microscopy and confocal microscopy. The ribosomal protein SA (RPSA) elastin receptor was identified after affinity chromatography by western

SUBMITTER: Brassart B 

PROVIDER: S-EPMC6461924 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature