NanoSIMS single cell analyses reveal the contrasting nitrogen sources for small phytoplankton.

ABSTRACT: Nitrogen (N) is a limiting nutrient in vast regions of the world's oceans, yet the sources of N available to various phytoplankton groups remain poorly understood. In this study, we investigated inorganic carbon (C) fixation rates and nitrate (NO₃^-), ammonium (NH₄⁺) and urea uptake rates at the single cell level in photosynthetic pico-eukaryotes (PPE) and the cyanobacteria Prochlorococcus and Synechococcus. To that end, we used dual ¹⁵N and ¹³C-labeled incubation assays coupled to flow cytometry cell sorting and nanoSIMS analysis on samples collected in the North Pacific Subtropical Gyre (NPSG) and in the California Current System (CCS). Based on these analyses, we found that photosynthetic growth rates (based on C fixation)

SUBMITTER: Berthelot H 

PROVIDER: S-EPMC6461931 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature