Cell-free miR-17-5p as a diagnostic biomarker for gastric cancer inhibits dendritic cell maturation.

ABSTRACT:

Purpose

Gastric cancer (GC) patients display aberrant miRNA expression and defective dendritic cell function. However, the role of cancer cell-derived oncomiR in GC detection and dendritic cell (DC) maturation remains largely elusive.

Methods

Candidate miRNAs were selected by deep sequencing (8 GC plasma samples vs 8 control plasma samples; 8 GC tissues vs 8 adjacent normal gastric tissues) and confirmed by PCR with 164 plasma samples and 72 formalin-fixed paraffin-embedded GC tissue samples. Their diagnostic performance was evaluated by receiver operating characteristic curve. Cy3 fluorescence signals in DCs, exposed to conditioned medium obtained from BGC-823 cells pre-transfected with Cy3-miR-17-5p, were determined by flow cytometry and visualized by confocal microscopy.

SUBMITTER: Cui ZJ 

PROVIDER: S-EPMC6462162 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature