The human lung mucosa drives differential Mycobacterium tuberculosis infection outcome in the alveolar epithelium.

ABSTRACT: Mycobacterium tuberculosis (M.tb) is deposited into the alveolus where it first encounters the alveolar lining fluid (ALF) prior contacts host cells. We demonstrated that M.tb-exposure to human ALF alters its cell surface, driving better M.tb infection control by professional phagocytes. Contrary to these findings, our results with non-professional phagocytes alveolar epithelial cells (ATs) define two distinct subsets of human ALFs; where M.tb exposure to Low (L)-ALF or High(H)-ALF results in low or high intracellular bacterial growth rates in ATs, respectively. H-ALF exposed-M.tb growth within ATs was independent of M.tb-uptake, M.tb-trafficking, and M.tb-infection induced cytotoxicity; however, it was associated with enhanced bacterial replication within LAMP-1⁺/ABCA1⁺

SUBMITTER: Scordo JM 

PROVIDER: S-EPMC6462240 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature