Extracellular matrix signaling activates differentiation of adult ovary-derived oogonial stem cells in a species-specific manner.

ABSTRACT:

Objective

To test if ovarian microenvironmental cues affect oogonial stem cell (OSC) function in a species-specific manner.

Design

Animal and human study.

Setting

Research laboratory.

Patient(s)/animal(s)

Human ovarian cells obtained from cryopreserved ovarian cortical tissue of reproductive-age women, and ovarian cells and tissues from female C57BL/6 mice.

Intervention(s)

Mouse ovarian tissue, mouse OSCs (mOSCs) and human OSCs (hOSCs) were analyzed for extracellular matrix (ECM) protein expression, and OSCs isolated from adult mouse and human ovaries were cultured in the absence or presence of ECM proteins without or with an integrin signaling inhibitor.

Main outcome measure(s)

Gene expression and in vitro derived (IVD) oocyte formation.

Resul

SUBMITTER: MacDonald JA 

PROVIDER: S-EPMC6462309 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature