Encrustations on ureteral stents from patients without urinary tract infection reveal distinct urotypes and a low bacterial load.

ABSTRACT:

Background

Current knowledge of the urinary tract microbiome is limited to urine analysis and analysis of biofilms formed on Foley catheters. Bacterial biofilms on ureteral stents have rarely been investigated, and no cultivation-independent data are available on the microbiome of the encrustations on the stents.

Results

The typical encrustations of organic and inorganic urine-derived material, including microbial biofilms formed during 3-6 weeks on ureteral stents in patients treated for kidney and ureteral stones, and without reported urinary tract infection at the time of stent insertion, were analysed. Next-generation sequencing of the 16S rRNA gene V3-V4 region revealed presence of different urotypes, distinct bacterial communities. Analysis of bacterial load was perfor

SUBMITTER: Buhmann MT 

PROVIDER: S-EPMC6462311 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature