Cell Kinetics in the Adult Neurogenic Niche and Impact of Diet-Induced Accelerated Aging.

ABSTRACT: Neurogenesis in the adult brain, a powerful mechanism for neuronal plasticity and brain repair, is altered by aging and pathological conditions, including metabolic disorders. The search for mechanisms and therapeutic solutions to alter neurogenesis requires understanding of cell kinetics within neurogenic niches using a high-throughput quantitative approach. The challenge is in the dynamic nature of the process and multiple cell types involved, each having several potential modes of division or cell fate. Here we show that cell kinetics can be revealed through a combination of the BrdU/EdU pulse-chase, based on the circadian pattern of DNA replication, and a differential equations model that describes time-dependent cell densities. The model is validated through the analysis of cell kinet

SUBMITTER: Stankiewicz AJ 

PROVIDER: S-EPMC6462444 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature