Importance of a tRNA anticodon loop modification and a conserved, noncanonical anticodon stem pairing in tRNACGGProfor decoding

ABSTRACT: Modification of anticodon nucleotides allows tRNAs to decode multiple codons, expanding the genetic code. Additionally, modifications located in the anticodon loop, outside the anticodon itself, stabilize tRNA–codon interactions, increasing decoding fidelity. Anticodon loop nucleotide 37 is 3′ to the anticodon and, in tRNACGGPro, is methylated at the N1 position in its nucleobase (m¹G37). The m¹G37 modification in tRNACGGPro stabilizes its interaction with the codon and maintains the mRNA frame. However, it is unclear how m¹G37 affects binding at the decoding center to both cognate and +1 slippery codons. Here, we show that the tRNACGGProm¹G37 modification is important for the association step during binding to a cognate CCG codon. In contrast, m

SUBMITTER: Nguyen HA 

PROVIDER: S-EPMC6462517 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature