The mode of anesthesia influences outcome in mouse models of arterial thrombosis.

ABSTRACT:

Background

Arterial thrombosis models are important for preclinical evaluation of antithrombotics but how anesthetic protocol can influence experimental results is not studied.

Objectives

We studied how three most commonly used rodent anesthetics affect the induction of thrombosis and thrombus resolution with antiplatelet agent integrilin (Eptifibatide).

Methods

The Folts, electrolytic, and FeCl₃ models of carotid artery thrombosis were evaluated. The extent of blood flow reduction required to elicit cyclic flow reductions (CFR) was examined in the Folts model. The occlusion time and stability following electrolytic or FeCl₃ injury was assessed. The efficacy of Eptifibatide was studied in each cohort and clot composition following FeCl₃

SUBMITTER: Sashindranath M 

PROVIDER: S-EPMC6462741 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature