Shear stress and platelet-induced tensile forces regulate ADAMTS13-localization within the platelet thrombus.

ABSTRACT:

Background

The multimeric glycoprotein von Willebrand factor (VWF) mediates platelet adhesion and aggregation at the site of vessel injury. The adhesive activity of VWF is influenced by its multimer length which is regulated by the metalloprotease ADAMTS13. The ability of ADAMTS13 to regulate platelet thrombus growth in a shear-dependent manner has been described, however, the mechanistic basis of this action has not been well characterized.

Methods

We developed an mCherry-tagged murine ADAMTS13 protein and utilized an ex vivo flow chamber system to visualize the localization of ADAMTS13 within the platelet thrombus under different conditions of shear. Using this system, we also assessed the influence of platelet-mediated tensile force on ADAMTS13 localization within the thr

SUBMITTER: Shida Y 

PROVIDER: S-EPMC6462754 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature